<?xml version='1.0' encoding='utf-8'?>
<emdEntry accessCode="0621" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2019-06-05</lastUpdate>
    </admin>
    <deposition>
        <status>REL</status>
        <depositionDate>2019-03-01</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2019-03-27</headerReleaseDate>
        <mapReleaseDate>2019-06-05</mapReleaseDate>
        <title>Structure of the AAV2 with its Cell Receptor, AAVR</title>
        <authors>Hu GQ, Meyer NL, Stagg SM, Chapman MS, Davulcu O, Xie Q, Noble AJ, Yoshioka C, Gingerich D, Trzynka A, David L</authors>
        <primaryReference published="true">
            <journalArticle>
                <authors>Meyer NL, Hu G, Davulcu O, Xie Q, Noble AJ, Yoshioka C, Gingerich DS, Trzynka A, David L, Stagg SM, Chapman MS</authors>
                <articleTitle>Structure of the gene therapy vector, adeno-associated virus with its cell receptor, AAVR.</articleTitle>
                <journal>Elife</journal>
                <volume>8</volume>
                <year>2019</year>
                <externalReference type="pubmed">31115336</externalReference>
                <externalReference type="doi">doi:10.7554/eLife.44707</externalReference>
                <externalReference type="issn">2050-084X</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="187" type="map">emd_0621.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>36</numRows>
            <numColumns>36</numColumns>
            <numSections>36</numSections>
        </dimensions>
        <origin>
            <originRow>-18.0</originRow>
            <originCol>-18.0</originCol>
            <originSec>-18.0</originSec>
        </origin>
        <limit>
            <limitRow>17.0</limitRow>
            <limitCol>17.0</limitCol>
            <limitSec>17.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>36</spacingRow>
            <spacingCol>36</spacingCol>
            <spacingSec>36</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">561.60004</cellA>
            <cellB units="A">561.60004</cellB>
            <cellC units="A">561.60004</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-1.5940299</minimum>
            <maximum>2.6960394</maximum>
            <average>0.00052377983</average>
            <std>0.33621302</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">15.600001</pixelX>
            <pixelY units="A">15.600001</pixelY>
            <pixelZ units="A">15.600001</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.22</contourLevel>
        <annotationDetails>AAVR class 1 EM map</annotationDetails>
    </map>
    <sample>
        <numComponents>1</numComponents>
        <name>AAV2 complex with AAVR receptor</name>
        <details>class 1</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>AAV2 complex with AAVR receptor</sciName>
                <molWtTheo units="MDa">3.75</molWtTheo>
                <details>class 1</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="272636">Adeno-associated virus</sciSpeciesName>
                    <sciSpeciesStrain>hybrid of serotypes 2, 8, and 9</sciSpeciesStrain>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="7108">Spodoptera frugiperda</expSystem>
                        <expSystemStrain>SF9</expSystemStrain>
                        <expSystemCell>Sf9</expSystemCell>
                        <vector>pFBDDJM11</vector>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>100</humidity>
            <temperature units="Kelvin">4</temperature>
            <instrument>FEI VITROBOT MARK IV</instrument>
            <details />
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">1.42</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <nominalDefocusMin units="nm">9000.0</nominalDefocusMin>
            <nominalDefocusMax units="nm">11.0</nominalDefocusMax>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <details>preliminary grid screening was performed manually</details>
            <detector>DIRECT ELECTRON DE-20 (5k x 3k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <calibratedMagnification>18000.0</calibratedMagnification>
            <temperatureMin units="Kelvin">290.0</temperatureMin>
            <temperatureMax units="Kelvin">300.0</temperatureMax>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
            <nominalMagnification>18000.0</nominalMagnification>
        </imaging>
        <imageAcquisition>
            <samplingSize units="microns">10.0</samplingSize>
            <details>none</details>
        </imageAcquisition>
        <fitting>
            <pdbEntryIdList>
                <pdbEntryId>6NZ0</pdbEntryId>
            </pdbEntryIdList>
            <refProtocol>rigid body</refProtocol>
            <targetCriteria>correlation coeeficient</targetCriteria>
            <refSpace>REAL</refSpace>
        </fitting>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">0.6</specimenConc>
            <buffer>
                <ph>7.4</ph>
                <details>blot force = 1, blot time = 3 seconds, total blots = 1</details>
            </buffer>
            <specimenSupportDetails>none</specimenSupportDetails>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>subtomogramAveraging</method>
        <reconstruction>
            <algorithm>BACK PROJECTION</algorithm>
            <software>Dynamo</software>
            <ctfCorrection>tomoctf</ctfCorrection>
            <resolutionByAuthor>20.0</resolutionByAuthor>
            <resolutionMethod>OTHER</resolutionMethod>
            <details>none</details>
            <eulerAnglesDetails>none</eulerAnglesDetails>
        </reconstruction>
        <subtomogramAveraging>
            <appliedSymmetry>I</appliedSymmetry>
            <numSubtomograms>1321</numSubtomograms>
            <numClassAverages>20</numClassAverages>
            <details>nond</details>
        </subtomogramAveraging>
    </processing>
</emdEntry>