<?xml version="1.0" encoding="UTF-8"?>
<emdEntry version="1.9.6" accessCode="6708">
    <admin>
        <lastUpdate>2017-12-20</lastUpdate>
    </admin>
    <deposition>
        <status>REL</status>
        <depositionDate>2017-02-22</depositionDate>
        <depositionSite>PDBj</depositionSite>
        <processingSite>PDBj</processingSite>
        <headerReleaseDate>2017-12-20</headerReleaseDate>
        <mapReleaseDate>2017-12-20</mapReleaseDate>
        <title>Intact ATR/Mec1-ATRIP/Ddc2 complex</title>
        <authors>Wang X, Ran T</authors>
        <fittedPDBEntryIdList>
            <fittedPDBEntryId>5x6o</fittedPDBEntryId>
        </fittedPDBEntryIdList>
        <primaryReference published="true">
            <journalArticle>
                <authors>Wang X, Ran T, Zhang X, Xin J, Zhang Z, Wu T, Wang W, Cai G</authors>
                <articleTitle>3.9 angstrom structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP.</articleTitle>
                <journal>Science</journal>
                <volume>358</volume>
                <firstPage>1206</firstPage>
                <lastPage>1209</lastPage>
                <year>2017</year>
                <externalReference type="pubmed">29191911</externalReference>
                <externalReference type="doi">doi:10.1126/science.aan8414</externalReference>
                <externalReference type="issn">1095-9203</externalReference>
                <externalReference type="csd">0038</externalReference>
                <externalReference type="astm">SCIEAS</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file sizeKb="67109" type="map" format="CCP4">emd_6708.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>256</numRows>
            <numColumns>256</numColumns>
            <numSections>256</numSections>
        </dimensions>
        <origin>
            <originRow>0</originRow>
            <originCol>0</originCol>
            <originSec>0</originSec>
        </origin>
        <limit>
            <limitRow>255</limitRow>
            <limitCol>255</limitCol>
            <limitSec>255</limitSec>
        </limit>
        <spacing>
            <spacingRow>256</spacingRow>
            <spacingCol>256</spacingCol>
            <spacingSec>256</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">345.6</cellA>
            <cellB units="A">345.6</cellB>
            <cellC units="A">345.6</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.15051116</minimum>
            <maximum>0.24395004</maximum>
            <average>0.000500057</average>
            <std>0.010790058</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details></details>
        <pixelSpacing>
            <pixelX units="A">1.35</pixelX>
            <pixelY units="A">1.35</pixelY>
            <pixelZ units="A">1.35</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.0518</contourLevel>
    </map>
    <sample>
        <numComponents>3</numComponents>
        <name>ATR/Mec1-ATRIP/Ddc2</name>
        <details>ATR/Mec1 "butterfly"</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>ATR/Mec1-ATRIP/Ddc2</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="4932">Saccharomyces cerevisiae</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <natSource/>
                    <details>ATR/Mec1 "butterfly"</details>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>Serine/threonine-protein kinase MEC1</sciName>
                <molWtTheo units="MDa">0.273680812</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="559292">Saccharomyces cerevisiae (strain ATCC 204508 / S288c)</sciSpeciesName>
                    <sciSpeciesStrain>ATCC 204508 / S288c</sciSpeciesStrain>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <natSource/>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>DNA damage checkpoint protein LCD1</sciName>
                <molWtTheo units="MDa">0.086533594</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="559292">Saccharomyces cerevisiae (strain ATCC 204508 / S288c)</sciSpeciesName>
                    <sciSpeciesStrain>ATCC 204508 / S288c</sciSpeciesStrain>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <natSource/>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">30.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>GATAN K2 (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition/>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>8.</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <resolutionByAuthor>3.9</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <numProjections>63132</numProjections>
        </singleParticle>
    </processing>
</emdEntry>
